Gas chromatograph injection agịgana-ejikarị 1ul na 10ul. Ọ bụ ezie na agịga injection dị ntakịrị, ọ dị mkpa. agịga injection bụ ọwa na-ejikọta sample na ngwa nyocha. Site na agịga injection, ihe nlele ahụ nwere ike ịbanye na kọlụm chromatographic wee gafee ihe nchọta maka nyocha ụdịdị dị iche iche na-aga n'ihu. Ya mere, mmezi na ihicha nke agịga injection bụ ihe ndị nyocha na-elekwasị anya kwa ụbọchị. Ma ọ bụghị ya, ọ bụghị naanị na ọ ga-emetụta arụmọrụ ọrụ, kamakwa na-emebi ngwá ọrụ ahụ. Ọnụ ọgụgụ na-esonụ na-egosi akụkụ nke agịga injection.
Nhazi nke agịga injection
Dị ka agịga ịgba ahụ si dị, a pụrụ ikewa ya gaa na agịga ịgba ogwu conical, agịga agịga bevel, na agịga injection dị larịị. A na-eji agịga conical eme ihe maka injection septum, nke nwere ike ibelata mmebi nke septum ma nagide ọtụtụ injections. A na-ejikarị ha eme ihe na injectors akpaka; Enwere ike iji agịga bevel mee ihe na septa injection, nke dị mfe iji rụọ ọrụ. N'ime ha, agịga 26s-22 kacha mma maka ojiji na septa injection na chromatography gas; A na-ejikarị agịga injection dị larịị na valvụ injection na pipettes nke chromatographs mmiri na-arụ ọrụ dị elu.
Dị ka usoro ịgba ntụtụ si dị, enwere ike kewaa ya ka ọ bụrụ agịga injection akpaaka na mkpịsị akwụkwọ ntuziaka.
Dị ka nyocha dị iche iche chọrọ nke agịga injection na gas chromatograph na mmiri chromatograph mmiri mmiri, enwere ike kewaa ya na agịga injection gas na mmiri mmiri injection. Ngwunye ogbugba chromatography gas na-achọkarị ntanye ntanye, yana olu injection kachasị bụ 0.2-1ul, yabụ agịga injection kwekọrọ na-abụkarị 10-25ul. agịga ahọpụtara bụ agịga ụdị cone, nke dị mma maka ịgba ọgwụ mgbochi; N'iji ya tụnyere, mmiri ọgwụ chromatography injection olu bụ n'ozuzu ibu, na nkịtị injection olu bụ 0.5-20ul, otú ikwu olu olu bụkwa ibu, n'ozuzu 25-100UL, na agịga n'ọnụ bụ ewepụghị iji gbochie scratching na stator.
Na nyocha chromatographic, agịga injection a na-ejikarị bụ agịga injection micro, nke dabara adaba maka chromatograph gas na nyocha mmiri chromatograph mmiri mmiri. Njehie ikike ya zuru oke bụ ± 5%. Arụmọrụ ikuku na-akwado 0.2Mpa. E kewara ya ụzọ abụọ: injector nchekwa mmiri na injector nchekwa mmiri. Ụdị nkọwapụta nke micro-injector na-abụghị mmiri mmiri bụ 0.5μL-5μL, na nkọwapụta nke micro-injector nke mmiri mmiri bụ 10μL-100μL. Agịga obere injection bụ ngwa dị oke mkpa dị mkpa.
Ojiji nke injector
(1) Lelee injector tupu eji ya, lelee ma sirinji ahụ ọ nwere mgbawa yana ma agbawara ọnụ agịga ahụ.
(2) Wepụ ihe fọdụrụ n'ime injector, sachaa injector na ihe mgbaze ugboro 5 ~ 20, ma tụfuo mmiri mmiri n'efu site na nke mbụ 2 ~ 3 ugboro.
(3) Wepụ ihe ndị dị na injector, gbanye agịga n'ime ihe mgbaze, ma see ihe nlele ahụ ugboro ugboro. Mgbe ị na-agbapụta ihe nlele ahụ, afụ dị na injector nwere ike ịgbanwe site na mgbanwe kwụ ọtọ nke tube.
(4) Mgbe ị na-eji injector, buru ụzọ jupụta mmiri mmiri ahụ injector, wee kpochaa mmiri ahụ na olu injection achọrọ.
Nlekọta nke agịga injection
(1) Ekwesịrị ịgbanye ihe nlele nke ọkara ruo elu viscosity ma ọ bụ buru nnukwu agịga ịgba ntụtụ dị n'ime ya tupu ejiri ya.
(2) Mgbe ị na-ehicha agịga, a ga-eji ngwá ọrụ ihicha ihe, dị ka waya nduzi ma ọ bụ stylet, tweezers, na surfactants kwesịrị iji hichaa mgbidi agịga.
(3) Nhicha ọkụ: A na-eji ihicha ọkụ na-ekpochapụ ihe ndị dị ndụ na agịga, karịsịa maka nyocha nyocha, isi ihe na-esi ísì ụtọ na ihe ndị na-egbuke egbuke. Mgbe nkeji ole na ole nke ihicha ọkụ, enwere ike iji ihe nhicha agịga mee ihe ọzọ.
Nhicha nke ogwu ogwu
1. Enwere ike ihicha mgbidi dị n'ime nke agịga injection na ihe mgbaze organic. Mgbe ị na-ehicha ihe, biko lelee ma mkpanaka ịgbanye agịga nwere ike ịga nke ọma;
2. Ọ bụrụ na mkpanaka ịgbanye agịga injection adịghị aga nke ọma, enwere ike iwepụ mkpanaka ahụ. A na-atụ aro ka ihichapụ ya dị ọcha na akwa dị nro na-etinye na ihe mgbaze organic.
3. Ugboro ugboro na-eji organic mgbaze ka aspirate. Ọ bụrụ na nguzogide nke agịga na-atụgharị agịga na-abawanye ngwa ngwa mgbe ọtụtụ ọchịchọ gasịrị, ọ pụtara na a ka nwere obere unyi. N'okwu a, ọ dị mkpa ka emegharịa usoro nhicha ahụ.
4. Ọ bụrụ na mkpara ịkwanye agịga injection nwere ike ịgagharị nke ọma na nwayọ, lelee ma akpọchiela agịga ahụ. Jiri ihe mgbaze organic sachaa agịga ahụ ugboro ugboro wee lelee udi nke ihe nlele a na-achụpụ na agịga ahụ.
5. Ọ bụrụ na agịga injection bụ ihe nkịtị, ihe nlele ahụ ga-apụta n'ahịrị kwụ ọtọ. Ọ bụrụ na agịga kpuchiri, a ga-efesa ihe nlele ahụ na ezigbo alụlụ site n'otu akụkụ ma ọ bụ akụkụ. Ọbụlagodi na ihe mgbaze na-apụta n'ahịrị kwụ ọtọ mgbe ụfọdụ, kpachara anya ka ị chọpụta na mmiri na-erugharị ahụ dị mma karịa ka ọ dị (naanị jiri ihe mgbaze ahụ tụnyere agịga ntụtụ ọhụrụ na-enweghị mgbochi).
6. Mgbochi dị na agịga ga-ebibi mmeghari nke nyocha. N'ihi nke a, mmezi agịga dị mkpa. Jiri ihe dị ka waya wepụ ihe mgbochi na agịga. Enwere ike iji agịga ahụ naanị mgbe nlele ahụ na-apụ apụ nke ọma. Iji pipette na-achọ mmiri mmiri ma ọ bụ ihe nchacha sirinji nwekwara ike wepụ ihe ndị na-emerụ emerụ na agịga nke ọma.
Akpachara anya mgbe ị na-eji agịga injection
Ejidela agịga sirinji na akụkụ sample na aka gị, ma enwekwaghị afụ (mgbe ị na-achọsi ike, gbanye nwayọọ nwayọọ, ngwa ngwa, wee jiri nwayọọ nwayọọ na-emegharị ugboro ugboro, 10 Olu nke agịga ígwè nke sirinji μl bụ 0.6). μl. Ọ bụrụ na e nwere afụ, ị gaghị ahụ ha, were 1-2μl ọzọ wee tụọ aka n'ọnụ agịga elu ruo mgbe afụ na-aga n'elu, wee gbanye mkpanaka agịga iji wepụ ihe ndị ahụ. sirinji nwere isi na-adị larịị) Ọsọ ịgba ntụtụ kwesịrị ịdị ngwa ngwa (ma ọ bụghị ngwa ngwa), debe otu ọsọ ahụ maka ntụtụ ọ bụla, wee malite ịgbanye ihe nlele ahụ mgbe ọnụ agịga rutere n'etiti ọnụ ụlọ vaporization.
Kedu ka esi egbochi agịga ịgba ntụtụ ka ọ gbadaa? Ọtụtụ ndị novice na-eme nyocha chromatography na-ehulata agịga na mkpanaka sirinji nke sirinji. Ihe kpatara ya bụ:
1. A na-akụda ọdụ ụgbọ mmiri injection nke ukwuu. Ọ bụrụ na akpọchie ya nke ọma na ụlọ okpomọkụ, akara silicone ga-agbasawanye ma sie ike mgbe okpomọkụ nke ụlọ vaporization na-ebili. N'oge a, ọ na-esiri ike itinye sirinji.
2. A na-arapara agịga ahụ n'ime akụkụ ígwè nke ọdụ ụgbọ mmiri ahụ mgbe a na-ahụghị ọnọdụ ahụ nke ọma.
3. A na-ehulata mkpanaka sirinji n'ihi na a na-eji ike dị ukwuu n'oge ịgba ọgwụ. Ọmarịcha, chromatograph ndị a na-ebubata na-abịa na akwa injector, na ịgbanye igwe injector agaghị ehulata mkpanaka sirinji.
4. N'ihi na mgbidi dị n'ime nke sirinji metọọ, a na-agbanye mkpanaka agịga ma na-ehulata n'oge injection. Mgbe ị na-eji sirinji mee ihe ruo oge ụfọdụ, ị ga-ahụ obere ihe ojii dị n'akụkụ elu nke agịga agịga, ọ ga-esikwa ike ịmịpụta ihe nlele na ịgbanye. Usoro nhicha: Wepụ mkpanaka agịga ahụ, gbanye ntakịrị mmiri, tinye mkpanaka agịga n'ime ọnọdụ emerụrụ ma gbanye ma dọpụta ugboro ugboro. Ọ bụrụ na ọ naghị arụ ọrụ otu ugboro, gbanye mmiri ọzọ ruo mgbe ewepụrụ ihe mmetọ ahụ. N'oge a, ị ga-ahụ na mmiri dị na sirinji na-aghọ turbid. Wepụ mkpanaka agịga ahụ ma hichaa ya na akwụkwọ nzacha, wee saa ya na mmanya na-aba n'anya ọtụtụ oge. Mgbe ihe nlele a ga-enyocha bụ ihe nlele siri ike gbasasịa n'ime ihe mgbaze, were ihe mgbaze saa sirinji ahụ n'oge ka agbachara ya.
5. Jide n'aka na ị kwụ ọtọ mgbe ị na-agba ọgwụ. Ọ bụrụ na ọ na-agụ gị agụụ ime ngwa ngwa, a ga-ehutu sirinji ahụ. Ọ bụrụhaala na ị maara nke ọma n'ịgba ọgwụ, ọ ga-adị ngwa ngwa.
Oge nzipu: Jun-19-2024