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Ụzọ isii iji hichaa ihe nlele HPLC

Biko mee nhọrọ nke gị dabere na ọnọdụ ụlọ nyocha nke gị.

Ọ dị mkpa ịchọta ụzọ dị irè isi hichaa vial sample

Ka ọ dị ugbu a, e nwere ọnụ ọgụgụ buru ibu nke ihe atụ nke ngwaahịa ọrụ ugbo (ihe ndị ọzọ kemịkalụ, organic acids, wdg) nke kwesịrị ịnwale site na chromatography mmiri mmiri na chromatography gas kwa afọ.N'ihi ọnụ ọgụgụ dị ukwuu nke ihe atụ, e nwere ọnụ ọgụgụ dị ukwuu nke ihe nlele nke dị mkpa ka ihichapụ n'oge usoro nchọpụta, nke na-eme ka ọ bụghị nanị na-egbu oge ma na-ebelata ọrụ ọrụ, ma mgbe ụfọdụ, ọ na-akpata ntụgharị na nsonaazụ nnwale n'ihi ịdị ọcha nke vials sample kpochara.

ASVSAV

Ihe nlere chromatographic bụ nke enyo na-abụkarị, a na-ejikarị plastik eme ya.Vial sample nke a na-atụfu ihe dị oke ọnụ, na-emebi emebi, na-emerụkwa gburugburu ebe obibi.Ọtụtụ ụlọ nyocha na-ehicha vial ihe nlele ma jiri ya mee ihe ọzọ.

Ka ọ dị ugbu a, ụzọ ụlọ nyocha a na-ejikarị eme ihe bụ nke a na-agbakwunye akwa, ncha, ihe mgbaze organic na ịsa acid, wee dozie obere usoro tube.

Usoro ịhịa ahụ a na-emekarị nwere ọtụtụ ọghọm:
Iji ncha na-eri mmiri nke ukwuu, oge ịsa ahụ na-adị ogologo, ma enwere akụkụ nke siri ike ihicha.Ọ bụrụ na ọ bụ ihe nlele plastik, ọ dị mfe ịnweta akara nrịanrịa n'ime mgbidi vials, nke na-ewe ọtụtụ ihe ọrụ.Maka iko iko nke a na-emetọ nke ukwuu site na lipid na protein residues, a na-eji ngwọta alkaline lysis eme ihe maka nhicha, na-enwetakwa nsonaazụ dị mma.

Mgbe ị na-enyocha ihe nlele site na LC/MS/MS, nhicha nke vials injection dị ezigbo mkpa.Dị ka usoro nhicha nke iko iko, a na-ahọrọ usoro nhicha dị ka ogo mmetọ si dị.Enweghị ụdị edozi.Nchịkọta usoro:

Nhọrọ nke Mbụ:

1. Wunye ngwọta ule na vials akọrọ
2. Ghichaa ihe ngwọta ule niile na mmanya 95%, na-asa ya ugboro abụọ na ultrasonic ma wụsa ya, n'ihi na mmanya na-aba n'anya na-abanye ngwa ngwa 1.5mL vial ma nwee ike ịmegharị ya na ọtụtụ ihe mgbaze organic iji nweta mmetụta nhicha.
3. Wunye na mmiri dị ọcha, na ultrasonically saa ugboro abụọ.
4. Wunye ude mmiri na vials akọrọ na akpọọ nkụ na 110 degrees Celsius maka 1 ruo 2 awa.Emela achịcha na okpomọkụ dị elu.
5. Jụụ ma chekwaa.

Nhọrọ nke abụọ:

1. Jiri mmiri mgbata kpochaa ọtụtụ ugboro
2. Tinye ya na beaker jupụtara na mmiri dị ọcha (Millipore pure water machine) na sonicate maka nkeji iri na ise.
3. Gbanwee mmiri na ultrasound maka nkeji iri na ise
4. Sook na beaker jupụtara ethanol zuru oke (Sinopharm Group, Analytical Pure)
5. N'ikpeazụ, wepụ ya ma hapụ ya ka ọ kpọọ nkụ.

Nhọrọ atọ:

1. Mbụ sook na methanol (chromatographically dị ọcha), na ultrasonically dị ọcha maka nkeji 20, wee wụsa methanol asat.
2. Jupụta ihe atụ vials na mmiri, na ultrasonically dị ọcha maka nkeji 20, wụsa mmiri.
3. Ghichaa vial sample ma emechaa.

Nhọrọ nke anọ:

Usoro ịsacha nke vials sample bụ otu ihe ahụ dị ka nkwadebe nke mmiri mmiri mmiri, wdg. Nke mbụ, jiri mmanya na-egbu egbu na-aṅụ ọgwụ maka ihe karịrị awa 4, mgbe ahụ, ultrasound maka ọkara otu awa, wee wụpụ mmanya na-aba n'anya ahụike, ma jiri mmiri mee ihe. maka ọkara nke ultrasound.Oge awa, kpochaa na mmiri ma kpoo ya.

Nhọrọ ise:

Nke mbụ, gbanye na ngwọta oxidizing siri ike (potassium dichromate) maka awa 24, wee jiri mmiri deionized na ultrasonic saa ya ugboro atọ n'okpuru ọnọdụ ahụ, n'ikpeazụkwa na-asa ya na methanol otu ugboro, wee kpoo ya maka ojiji.
A ghaghị dochie septas caps, karịsịa mgbe ị na-enyocha ihe fọdụrụ na pesticide, ma ọ bụghị ya, ọ ga-emetụta ọnụọgụgụ.
Ma ọ bụrụ na ọnọdụ kwenye, gbalịa iji ihe ndị a na-ekpofu ọkụ, dị ka ihe ntinye PTFE ma ọ bụ ihe ntinye plastik nke ụlọ (ihe dị ka 0.1 yuan / ibe), na ihe nlele nlele dị mma.Ojiji ugboro ugboro na ọ dịghị mkpa ka ihicha ya.

Nhọrọ nke isii:

(1) usoro nhicha dị mgbagwoju anya nwere nsonaazụ bara uru:
Mba1.Mgbe ejiri ihe nlele ahụ mee ihe, kpochapụ ihe nlele ahụ na mmiri na-agba ọsọ na mbụ, ma kpochapụ ihe fọdụrụ (ị nwere ike ịkwanye ya n'aka n'otu oge);
No2, tinyezie vials sample n'ime potassium dichromate na-asacha mmiri mmiri afụ, na mgbe ọ na-akwakọba Mgbe ị ruru ole ma ọ bụ mgbe obi dị gị mma, wepụ ya na tank mmiri ma tinye ya na sieve plastik maka kichin. jiri.Jiri mmiri mgbata kpochaa nke ọma.Ị nwere ike sieve ugboro ugboro ma maa jijiji n'etiti;
Mba 3.Jiri mmiri mgbata mee ka ultrasonically dị ọcha ugboro 3 mgbe ịsachara.Gburugburu, ọ kacha mma ịkwanye mmiri na vials sample mgbe ọ bụla ultrasonic ihicha;
No4, wee jiri mmiri na-ekpo ọkụ okpukpu atọ (ma ọ bụ mmiri dị ọcha, mmiri na-ekpo ọkụ) na 1.3 ultrasonic ihicha ugboro atọ;
No5, wee jiri chromatographic dị ọcha methanol ultrasonic ihicha 2-3 ugboro, ọ dịkwa mma ka
kpochapu methanol na vials sample mgbe ọ bụla ihichachara;
Mba 6.Tinye ihe nlele ahụ na oven wee kpoo ya na ihe dịka ogo 80, a pụkwara iji ya mee ihe.

(2) sample vial zụtara ka akara na agba dị iche iche:

ma ọ bụrụ na ị chọpụtala na e nwere obere akara agba na vials sample, nke na-abụghị maka ọdịdị dị mma, ma na-eji ya eme ihe.Mgbe ịzụrụ, ọ kacha mma ịzụta ọtụtụ vial nke agba dị iche iche.

Dịka ọmụmaatụ: ụlọ nyocha gị na-emepe ọrụ abụọ A na B n'otu oge.Oge mbụ A oru ngo na-eji vials ọcha, na B oru ngo na-eji a-acha anụnụ anụnụ sample vial.Mgbe emechara ule ahụ, a na-ehicha ya dị ka usoro a dị n'elu si dị, na nnwale nke abụọ n'oge ahụ, jiri vials na-acha anụnụ anụnụ maka ọrụ A, ihe nlele ọcha maka ọrụ B, na ihe ndị ọzọ, nke nwere ike izere nke ọma nsogbu kpatara. mmetọ maka ọrụ gị.

Dee na njedebe

1. Ọtụtụ ngwá injinia tụrụ aro: Jiri a muffle ọkụ na 400 degrees ka akpọọ nkụ maka ọkara otu awa, na organic ihe na-isi-apụ;
2. Tinye vial ihe atụ n'ime ọkụ muffle maka ihicha na 300 degrees Celsius.Onye injinia Agilent si Beijing kwuru na mgbe ọ bịara n'ọkụ muffle, ule ahụ agaghị abụ mkpọtụ mgbe emechara n'ọkụ muffle na ogo 300 maka awa 6.

nakwa………..
Enwere ike ihicha obere flask volumetric, flasks ndị yiri ube maka evaporation rotary, na iko ndị ọzọ maka nyocha ma ọ bụ pretreatment nwere ike ihicha site n'ịtụ aka na usoro a.

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Oge nzipu: Feb-25-2022