Usoro mkpanaaka dabara na akụkụ mmiri mmiri nke ọbara, na enwere ihe dị iche iche ị ga-aṅa ntị n'oge eji. N'ime ha, e nwere ụfọdụ "ọnyà" nke a ga-aṅa ntị na ya.
01. Tụọ pH nke mobile na-adọ mgbe agbakwunyere organic mgbaze
Ọ bụrụ na ị jiri ihe mgbakwunye organic tụọ pH, pH ị ga-enweta ga-adị iche karịa tupu ị gbakwunye ihe mgbaze organic. Otú ọ dị, ihe kasị mkpa bụ na-agbanwe agbanwe. Ọ bụrụ na ị na-atụ pH mgbe niile mgbe ị gbakwunyere ihe mgbaze organic, jide n'aka na ị ga-ekwupụta usoro gị na usoro ị na-eji ka ndị ọzọ wee soro otu usoro ahụ. Usoro a abụghị 100% ziri ezi, ma ọ dịkarịa ala ọ ga-edobe usoro ahụ. Nke a nwere ike ịdị mkpa karịa ịnweta uru pH ziri ezi.
02. Enweghị ihe nchekwa eji
Ebumnuche nke nchekwa bụ ijikwa pH ma gbochie ya ịgbanwe. Ọtụtụ ụzọ ndị ọzọ na-agbanwe pH nke usoro mkpanaaka, nke nwere ike ibute mgbanwe na oge njide, ọdịdị kacha elu, na nzaghachi kacha elu.
Formic acid, TFA, wdg abụghị ihe nchekwa
03. Anaghị eji nchekwa n'ime oke pH nkịtị
Ihe nchekwa ọ bụla nwere obosara nkeji pH 2, n'ime nke ọ na-enye nkwụsi ike pH kacha mma. Ihe nchekwa na-abụghị mpio a agaghị enye ezigbo nguzogide pH. Ma ọ bụ jiri ihe nchekwa na oke ziri ezi, ma ọ bụ họrọ ihe nchekwa nke na-ekpuchi oke pH ị chọrọ.
04. Tinye ihe nchekwa na ngwọta organic
Ịgwakọta ihe ngwọta na-edozi ahụ na akụkụ organic ga-eme ka ihe nchekwa ahụ mee ka ọ daa. N'ọtụtụ ọnọdụ, ọ bụrụgodị na mmiri ozuzo emeela, ọ ka na-esiri ike ịchọpụta. Cheta na ị na-agbakwunye ihe ngwọta organic mgbe niile na usoro mmiri mmiri, nke nwere ike belata ohere nke mmiri ozuzo.
05. Gwakọta gradient ntinye uche site na 0% na mgbapụta
Nfuli dị taa nwere ike ịgwakọta mobile n'ụzọ na degas inline, ma ọ bụghị onye ọ bụla na-eji gị usoro ga-enwe elu àgwà mgbapụta. Gwakọta A na B n'ime otu ngwọta ma mee ya 100% inline.
Dịka ọmụmaatụ, 950 ml nke ngwakọta mmalite organic nwere ike ịkwadebe site na ịgwakọta na 50 ml mmiri. Uru nke a bụ na ọ nwere ike belata mgbanwe dị n'etiti HPLC ma belata ohere nke afụ na mmiri ozuzo na usoro. Ọ bụ ihe kwesịrị ịrịba ama na ruru nke mgbapụta ngwakọta bụ 95: 5, nke a pụtaghị na tupu mixed oge njigide na karama bụkwa 95: 5.
06. Ọ bụghị iji acid gbanwetụrụ ziri ezi (base) iji gbanwee ihe nchekwa
Jiri naanị acid ma ọ bụ ntọala na-emepụta nnu na-ekpuchi ihe ị na-eji. Dịka ọmụmaatụ, a ga-eji naanị phosphoric acid ma ọ bụ sodium hydroxide kwadebe ihe nchekwa sodium phosphate.
07. Ọ bụghị ịkọwa ozi niile gbasara ihe nchekwa na usoro ahụ, dị ka ịgbakwunye 5g nkesodium phosphate ruo 1000 ml mmiri.
Ụdị ihe nkpuchi na-ekpebi oke pH nke enwere ike ịkwado. Ntinye uche achọrọ na-ekpebi ike nchekwa. gram 5 ma ọ bụ sodium phosphate anhydrous na gram 5 nke monosodium phosphate monohydrate nwere ikike nchekwa dị iche iche.
08. Ịgbakwunye organic solvents tupu ịlele
Ọ bụrụ na usoro gara aga jiri ngwọta nchekwa maka baseline B, na usoro gị na-eji ngwọta organic maka baseline B, ị nwere ike ịtụ anya dozie ihe nchekwa ahụ na tubing mgbapụta na isi mgbapụta.
09. Bulie karama ma kpofuo ndo ikpeazụ
Enwere ezigbo ohere na ị gaghị enwe usoro mkpanaka zuru oke iji mechaa ọsọ ahụ dum na nlele gị ga-ese anwụrụ. E wezụga ohere nke na-ere ọkụ na usoro mgbapụta na kọlụm, usoro mkpanaaka ga-apụ kpamkpam na usoro mkpanaka n'elu karama ahụ ga-agbanwe.
10. Jiri ultrasonic degassing mobile frasi
Ihe kachasị mkpa bụ ijide n'aka na agbazere salts niile na-echekwa, ma nke a bụ ụzọ kachasị njọ na degas na ọ ga-ekpo ọkụ ngwa ngwa ngwa ngwa, na-eme ka ihe ndị na-emepụta ihe na-ekpochapụ. Ka ịchekwaa nsogbu na-enweghị isi ma emechaa, were nkeji ise ka ihichapụ ihe nzacha ekwentị gị.
Oge nzipu: Ọgọst-27-2024